Ribosomal RNA synthesis and ribosome formation during germination of flax rust uredospores
Identifieur interne : 002019 ( Main/Exploration ); précédent : 002018; suivant : 002020Ribosomal RNA synthesis and ribosome formation during germination of flax rust uredospores
Auteurs : A. K. Chakravorty [Canada] ; Michael Shaw [Canada]Source :
- Physiological Plant Pathology [ 0048-4059 ] ; 1972.
English descriptors
- Teeft :
- Biochimica biophysics acta, Canadian journal, Chakravorty, Favourable medium, Flax, Flax rust, Flax rust uredospores, Fraction number, Germinating, Germinating flax rust uredospores, Germinating uredospores, Germination, Gramicidin, Incubation medium, Linear sucrose, Melampsora lini, Molecular biology, Monosomes, National academy, Nucleolus, Optical density, Polysomes, Protein synthesis, Radioactivity profile, Ribosomal, Ribosomal subunits, Ribosome, Ribosome formation, Rrna, Rrna synthesis, Sedimentation pattern, Sephadex column, Spore, Subunit, Sucrose, Sucrose gradient, Uredospores, Vigna unguiculata.
Abstract
Abstract: Ribosomal RNA (28 s, 18 s and 5 s rRNA) is synthesized in germinating uredospores of flax rust [Melampsora lini (Pers.) Lev., Race no. 3] during the initial 6 h of hydration in the absence of the host or of a favourable medium. During this period, the newly transcribed rRNA molecules are associated with mature ribosomal subunits (60 s and 40 s) and with monomeric ribosomes (80 s). The newly formed ribosomes, containing32P-labeled RNA and3H-labeled proteins, actively engage in protein synthesis in vivo since they were recovered from the polysomal region after monosomes and polysomes were separated by sucrose density-gradient centrifugation.
Url:
DOI: 10.1016/0048-4059(72)90042-2
Affiliations:
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- to stream Main, to step Curation: 002019
Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Ribosomal RNA (28 s, 18 s and 5 s rRNA) is synthesized in germinating uredospores of flax rust [Melampsora lini (Pers.) Lev., Race no. 3] during the initial 6 h of hydration in the absence of the host or of a favourable medium. During this period, the newly transcribed rRNA molecules are associated with mature ribosomal subunits (60 s and 40 s) and with monomeric ribosomes (80 s). The newly formed ribosomes, containing32P-labeled RNA and3H-labeled proteins, actively engage in protein synthesis in vivo since they were recovered from the polysomal region after monosomes and polysomes were separated by sucrose density-gradient centrifugation.</div>
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